Information
Home Algae's crosslinked APC provides a convenient way to conjugate Allophycocyanin (APC) to antibodies. APC belongs to the phycobiliproteins family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. APC, an ultra-sensitive fluorescent tracer with high emission quantum yields, emits in the near-infrared fluorescence range. It, therefore, avoids interference from autofluorescence of cellular components and other biological materials.
APC is significantly more sensitive than conventional organic fluorophores and has been used in applications such as Flow Cytometry, Homogeneous FRET assay, and Immunoassays.
Home Algae's crosslinked APC is an SH-reactive ready-to-use reagent. Modified Allophycocyanin has Maleimide groups that easily react with SH groups of a target antibody without the need for an additional activation.
Crosslinked APC
*with Ammonium Sulfate
Benefits of APC
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Ideal for multi-color fluorescent staining
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Applied to most fluorescence-based instruments
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Significant stokes shifts with resolvable emission spectra
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Ensure result accuracy
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Bright Index: Level 4 with high quantum yield
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It should be noted that the relatively high molecular weight of APC may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.
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Stability
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Minimal fluorescence quenching
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