Home Algae's crosslinked APC provides a convenient way to conjugate Allophycocyanin (APC) to antibodies. APC belongs to the phycobiliproteins family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. APC, an ultra-sensitive fluorescent tracer with high emission quantum yields, emits in the near-infrared fluorescence range. It, therefore, avoids interference from autofluorescence of cellular components and other biological materials.
APC is significantly more sensitive than conventional organic fluorophores and has been used in applications such as Flow Cytometry, Homogeneous FRET assay, and Immunoassays.
Home Algae's crosslinked APC is an SH-reactive ready-to-use reagent. Modified Allophycocyanin has Maleimide groups that easily react with SH groups of a target antibody without the need for an additional activation.
*with Ammonium Sulfate
Physical & Spectral Properties
Molecular weight: ~104 kDa
Absorption maximum (nm): 650
Emission maximum (nm): 660
> 10.0 mg/ml
Shipping & Handling
Standard overnight for Taiwan, inquire for International
UNSPSC Code: 12171501
Shelf Life and Storage Conditions
Shelf Life: 12 months from date of production
Storage Conditions: Refrigerate at 4°C, and keep in desiccated and dark upon arrival. Do not freeze!
Applied Instruments & Applications
Applied to most lab fluorescent-based instruments:
Flow Cytometry (FC)
Immunofluorescence Assay (IFA)
Circulating Tumor Cell (CTC) Research
- Cell Therapy
- ...and other laboratory researches
Benefits of APC
Ideal for multi-color fluorescent staining
Applied to most fluorescence-based instruments
Significant stokes shifts with resolvable emission spectra
Ensure result accuracy
Bright Index: Level 4 with high quantum yield
Won't penetrate into the cell and cause a false-positive reaction
Minimal fluorescence quenching
Non-toxic, less chance of affecting characteristics of the sample, human body, and environment