HomeALG™ Activated APC
HomeALG™ Activated APC

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HomeALG™ APC (Fluorescence Spectrum)
HomeALG™ APC (Fluorescence Spectrum)

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HomeALG™ Activated APC
HomeALG™ Activated APC

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Information

Home Algae's crosslinked APC provides a convenient way to conjugate Allophycocyanin (APC) to antibodies. APC belongs to the phycobiliproteins family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. APC, an ultra-sensitive fluorescent tracer with high emission quantum yields, emits in the near-infrared fluorescence range. It, therefore, avoids interference from autofluorescence of cellular components and other biological materials.

APC is significantly more sensitive than conventional organic fluorophores and has been used in applications such as Flow Cytometry, Homogeneous FRET assay, and Immunoassays.

 

Home Algae's crosslinked APC is an SH-reactive ready-to-use reagent. Modified Allophycocyanin has Maleimide groups that easily react with SH groups of a target antibody without the need for an additional activation.

Activated APC
*with Ammonium Sulfate

Physical & Spectral Properties


Molecular weight: ~104 kDa
Form: Liquid
Absorption maximum (nm): 650
Emission maximum (nm): 660




Specifications


Purity:
A650/A280>4.00
A650/A620>1.25

Concentration:
> 10.0 mg/ml




Shipping & Handling


Ship with cold pack, polystyrene foam container, shipping box

Standard overnight for Taiwan, inquire for International
HS Code: 32049000
UNSPSC Code: 12171501




Shelf Life and Storage Conditions


Shelf Life: 12 months from date of production
Storage Conditions: Refrigerate at 4°C, and keep in desiccated and dark upon arrival. Do not freeze!




Applied Instruments & Applications


Applied to most lab fluorescent-based instruments:

  • Flow Cytometry (FC)
  • Immunofluorescence Assay (IFA)
Can be used in researches such as:
  • Circulating Tumor Cell (CTC) Research
  • Cell Therapy
  • ...and other laboratory researches
*Note: This reagent is for research use only, not for diagnostic or therapeutic use.





Benefits of APC
  • Ideal for multi-color fluorescent staining

    • Applied to most fluorescence-based instruments

    • Significant stokes shifts with resolvable emission spectra

  • Ensure result accuracy

    • Bright Index: Level 4 with high quantum yield

    • Won't penetrate into the cell and cause a false-positive reaction

  • Stability

    • Minimal fluorescence quenching

    • Non-toxic, less chance of affecting characteristics of the sample, human body, and environment