FAQs

Phycobiliproteins (PBPs) stored in PBS with ammonium sulfate provide excellent long-term stability. However, this form requires additional purification steps, such as dialysis or sulfate precipitation, to remove ammonium sulfate before creating tandem dyes or antibody conjugations. We offer PBPs stored in PBS with ammonium sulfate for your convenience.

Our R-PE and APC Labeling Kits are SH-reactive, specifically designed to bind efficiently with free sulfhydryl (-SH) groups on antibodies. The maleimide (-NH2) groups in our crosslinked R-PE and APC create stable covalent bonds without requiring additional activation. These kits are also suitable for labeling other molecules with free sulfhydryl groups.

After labeling, the mixture mainly consists of the R-PE-antibody conjugate with minimal free R-PE and antibody. While free R-PE generally doesn't impact assay results, we recommend purification when labeling up to 1 mg of antibody for optimal performance. Reach out to us for technical support if needed.

Absolutely! Our labeling kits are designed to be scalable, allowing you to divide a large pack into smaller vials to suit your experimental needs.

Purified R-PE (240 kDa) and APC (105 kDa) are perfect for detecting cell surface antigens, aiding in cell screening and imaging. Their high molecular weights prevent penetration into cell samples, even if the cell membrane is ruptured, reducing false positives. R-PE is best for surface labeling in flow cytometry rather than penetrating cells or tissues.