Question 1

Are phycobiliproteins stored in PBS with ammonium sulfate different?

Accepted Answer

Phycobiliproteins (PBPs) stored in PBS with ammonium sulfate offer excellent long-term stability. However, this form requires additional purification steps, like dialysis or sulfate precipitation, to remove ammonium sulfate before creating tandem dyes or antibody conjugations. We provide PBPs stored in PBS with ammonium sulfate for your convenience.

Question 2

How do R-PE and APC conjugation reactions work with antibodies?

Accepted Answer

Our R-PE and APC Labeling Kits are SH-reactive, designed to bind efficiently with free sulfhydryl (-SH) groups on antibodies. The maleimide (-NH2) groups in our crosslinked R-PE and APC form stable covalent bonds without additional activation. These kits can also label other molecules with free sulfhydryl groups.

Question 3

Should I purify free R-PE from the R-PE-antibody conjugate after labeling?

Accepted Answer

After labeling, the mixture primarily contains the R-PE-antibody conjugate with minimal free R-PE and antibody. While free R-PE typically doesn't affect assay results, we recommend purification when labeling up to 1 mg of antibody for optimal performance. Contact us for technical support if needed.

Question 4

Can I divide a large labeling kit into smaller vials for multiple uses?

Accepted Answer

Yes, our labeling kits are scalable. You can divide a large pack into smaller vials to meet your experimental needs.

Question 5

What are the molecular weights of purified R-PE and APC, and their applications?

Accepted Answer

Purified R-PE (240 kDa) and APC (105 kDa) are perfect for detecting cell surface antigens, aiding in cell screening and imaging. Their high molecular weights prevent penetration into cell samples, even if the cell membrane is ruptured, reducing false positives. R-PE is best for surface labeling in flow cytometry rather than penetrating cells or tissues.

FAQs

Are phycobiliproteins stored in PBS with ammonium sulfate different?

How do R-PE and APC conjugation reactions work with antibodies?

Should I purify free R-PE from the R-PE-antibody conjugate after labeling?

Can I divide a large labeling kit into smaller vials for multiple uses?

What are the molecular weights of purified R-PE and APC, and their applications?

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