Product Information
Home Algae Biotech’s crosslinked R-PE, with a maleimide group introduced, allows it to be conveniently conjugated to the thiol groups of proteins, without the need for additional activation and purification, it facilitates the rapid PE conjugations to antibodies, other proteins, and reagents.
With a broad range of excitation wavelengths, R-PE can be easily excited by two laser sources (488 nm & 561 nm), applied to most lab fluorescence detection instruments i.e. Flow Cytometry, Microarray Assay, Immunofluorescence Assay (IFA). Home Algae's R-PE absorbance maxima are at 496, 546, and 565 nm, its significant stokes shifts with a resolvable emission peak at 578 nm.
Crosslinked R-PE
*Ready-to-use (Ammonium Sulfate-free)
Benefits of R-PE
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Ideal for multi-color fluorescent staining
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Can be excited by two laser sources, experiments can be performed without equipment upgrades
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Significant stokes shifts with resolvable emission spectra
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Multiple sites for stable conjugation with Biotin, Streptavidin, and other dyes, e.g., Cy-3, Cy-5, Cy-7
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Ensure result accuracy
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Bright Index: Level 5 with high quantum yield
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It should be noted that the relatively high molecular weight of R-PE may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.
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Stability
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Minimal fluorescence quenching
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