HomeALG™ R-PE Labeling Kit
HomeALG™ R-PE Labeling Kit

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HomeALG™ R-PE Labeled eBioscience™ mAb CD45
HomeALG™ R-PE Labeled eBioscience™ mAb CD45

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HomeALG™ R-PE Labeled CD45 antibody by IF
HomeALG™ R-PE Labeled CD45 antibody by IF

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HomeALG™ R-PE Labeling Kit
HomeALG™ R-PE Labeling Kit

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Information

Home Algae R-PE Labeling Kit is an SH-reactive labeling kit. Modified R-PE has maleimide groups that can conveniently conjugate with the SH groups of antibodies. The total conjugation time including incubation takes approximately 3 hours. 

 

With a broad range of excitation wavelengths, R-PE can be easily excited by two laser sources (488 nm & 561 nm), applied to most fluorescence detection instruments i.e. Flow Cytometry, Immunofluorescence Microscopy (IF), Microarray Assay. Home Algae's R-PE absorbance maxima are at 496, 545, and 565 nm, its significant stokes shifts with a resolvable emission peak at 578 nm

Sample of Protocol
  1. Add Modifier Solution into antibody

  2. Incubate the reaction at room temperature for 30 min

  3. Add Pre-activated R-PE and incubate 1 hour at room temperature with agitation and protect from light

  4. Add Quencher into the conjugation mixture and mix completely

  5. Incubate at room temperature and keep away from light for 30 min, the conjugation is complete.

Benefits of R-PE
  • Ideal for multi-color fluorescent staining

    • Can be excited by two laser sources, experiments can be performed without equipment upgrades

    • Significant stokes shifts with resolvable emission spectra

  • Ensure result accuracy

    • Bright Index: Level 5 with high quantum yield

    • Won't penetrate into the cell and cause a false-positive reaction

  • Stability

    • Minimal fluorescence quenching

    • Non-toxic, less chance of affecting characteristics of the sample, human body, and environment

R-PE Labeling Kit
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Physical & Spectral Properties


Molecular weight: ~240 kDa
Form: Liquid
Absorption maximum (nm): 496, 546, 565
Emission maximum (nm): 578




Specifications


Purity:
A566/A280>5.00
A566/A496<1.50
A620/A566<0.01




Shipping & Handling


Ship with cold pack, polystyrene foam container, shipping box

Standard overnight for Taiwan, inquire for International
HS Code: 32049000
UNSPSC Code: 12171501




Shelf Life and Storage Conditions


Shelf Life: 12 months from date of production
Storage Conditions: Refrigerate at 4°C, and keep in desiccated and dark upon arrival. Do not freeze.




Applied Instruments & Applications


Applied to most fluorescent-based instruments/detections:

  • Flow Cytometry (FC)
  • Immunofluorescence Microscopy (IF)
  • Microarrays
  • Molecular Assays
Can be used in researches such as:
  • Circulating Tumor Cell (CTC) Research
  • Cell Therapy
  • Apoptosis Detection
  • ...and other laboratory researches
*Note: This reagent is for research use only, not for diagnostic or therapeutic use.