HomeALG™ APC Labeling Kit
HomeALG™ APC Labeling Kit

press to zoom
HomeALG™ APC (Fluorescence Spectrum)
HomeALG™ APC (Fluorescence Spectrum)

press to zoom
HomeALG™ APC Labeling Kit
HomeALG™ APC Labeling Kit

press to zoom
Information

Home Algae's APC Labeling Kit is an SH-reactive labeling kit. Modified APC has maleimide groups that can conveniently conjugate with the SH groups of antibodies. The total conjugation time including incubation takes approximately 3 hours. 

 

APC belongs to the phycobiliproteins family of highly soluble and fluorescent proteins derived from cyanobacteria and eukaryotic algae. APC, an ultra-sensitive fluorescent tracer with high emission quantum yields, emits in the near-infrared fluorescence range. It, therefore, avoids interference from the autofluorescence of cellular components and other biological materials.

APC is significantly more sensitive than conventional organic fluorophores and has been used in applications such as Flow Cytometry, Homogeneous FRET assay, and Immunoassays.

Sample of Protocol
  1. Add Modifier Solution into antibody

  2. Incubate the reaction at room temperature for 30 min

  3. Add Pre-activated APC and incubate 1 hour at room temperature with agitation and protect from light

  4. Add Quencher into the conjugation mixture and mix completely

  5. Incubate at room temperature and keep away from light for 30 min, the conjugation is complete.

APC Labeling Kit
Fill out the form below to get a quote!
arrow&v

Your request has been submitted!

Physical & Spectral Properties


Molecular weight: 105 kDa
Form: Liquid
Absorption maximum (nm): 650
Emission maximum (nm): 660




Specifications


Purity:
A650/A280>4.00
A650/A620>1.25

Concentration:
> 10.0 mg/ml




Shipping & Handling


Ship with cold pack, polystyrene foam container, shipping box

Standard overnight for Taiwan, inquire for International
HS Code: 32049000
UNSPSC Code: 12171501




Shelf Life and Storage Conditions


Shelf Life: 12 months from date of production
Storage Conditions: Refrigerate at 4°C, and keep in desiccated and dark upon arrival. Do not freeze!




Applied Instruments & Applications


Applied to most lab fluorescent-based instruments:

  • Flow Cytometry (FC)
  • Immunofluorescence Assay (IFA)
Can be used in researches such as:
  • Circulating Tumor Cell (CTC) Research
  • Cell Therapy
  • ...and other laboratory researches
*Note: This reagent is for research use only, not for diagnostic or therapeutic use.





Benefits of APC
  • Ideal for multi-color fluorescent staining

    • Applied to most fluorescence-based instruments

    • Significant stokes shifts with resolvable emission spectra

  • Ensure result accuracy

    • Bright Index: Level 4 with high quantum yield

    • Won't penetrate into the cell and cause a false-positive reaction

  • Stability

    • Minimal fluorescence quenching

    • Non-toxic, less chance of affecting characteristics of the sample, human body, and environment