Question 1

Are phycobiliproteins stored in PBS only and in PBS with ammonium sulfate different?

Accepted Answer

PBPs in different buffer solutions both exhibit good long-term stability, the form with ammonium sulfate requires more purification processes, such as dialysis or sulfate precipitation, to fully remove the ammonium sulfate prior to making tandem dyes and antibody conjugations.
 
Home Algae Biotech offers phycobiliproteins stored in PBS only and PBS with ammonium sulfate buffer solutions.

Question 2

結合反應是否會將 R-PE/APC 與抗體上的遊離巰基連接起來？

Accepted Answer

Home Algae 的 R-PE 和 APC 標記試劑盒是 SH 反應標記試劑盒。交聯的 R-PE 和 APC 具有馬來酰亞胺 (-NH2) 基團，可輕鬆與目標抗體的巰基 (-SH) 基團反應，而無需額外的活化。 R-PE/APC的馬來酰亞胺基與抗體上的巰基在結合過程中形成共價鍵。 使用者還可以使用交聯的 R-PE 和 APC 來標記其他具有遊離巰基的分子。

Question 3

Do I need to remove the free R-PE from the R-PE-antibody conjugate?

Accepted Answer

After the conjugation procedures, reaction mixture should contain mostly the R-PE-antibody conjugate, with small amounts of unconjugated R-PE and antibody. Unconjugated R-PE should not interfere with the assay result. However, purification is suggested for labeling up to 1 mg of antibody. Please contact us for more technical advice.

Question 4

Can I purchase large pack of labeling kit and separate them into small vials on my own for several reactions?

Accepted Answer

Yes, all pack sizes of our labeling kits are scalable and can be separated into small packs based on your experiment requirements.

Question 5

What are the molecular weight of purified R-PE and APC?

Accepted Answer

The molecular weight of purified R-PE is 240 kDa, and for purified APC is 105 kDa.
 
They are both perfect for detecting cell surface antigens to further conduct cell screening and cell imaging. With high molecular weight, PE and APC won't penetrate into the cell sample even if cell membrane ruptured, which greatly lower the risk of false-positive reaction.
 
It should be noted that the relatively high molecular weight of R-PE may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.

FAQs

Are phycobiliproteins stored in PBS only and in PBS with ammonium sulfate different?

結合反應是否會將 R-PE/APC 與抗體上的遊離巰基連接起來？

Do I need to remove the free R-PE from the R-PE-antibody conjugate?

Can I purchase large pack of labeling kit and separate them into small vials on my own for several reactions?

What are the molecular weight of purified R-PE and APC?

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